High-throughput protein production and purification at the Seattle Structural Genomics Center for Infectious Disease
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High-throughput protein production and purification at the Seattle Structural Genomics Center for Infectious Disease
The establishment of an efficient and reliable protein-purification pipeline is essential for the success of structural genomic projects. The SSGCID Protein Purification Group at the University of Washington (UW-PPG) has established a robust protein-purification pipeline designed to purify 400 proteins per year at a rate of eight purifications per week. The pipeline was implemented using two ÄK...
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Robin Stacy,†,‡ Wayne F. Anderson, and Peter J. Myler*,†,‡,⊥ †Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, Washington 98109, United States ‡Seattle Structural Genomics Center for Infectious Disease, 307 Westlake Ave North, Suite 500, Seattle, Washington 98109, United States Department of Molecular Pharmacology and Biological Chemistry, Northwestern Unive...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section F Structural Biology and Crystallization Communications
سال: 2011
ISSN: 1744-3091
DOI: 10.1107/s1744309111018367